Molekula
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid)
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 1999 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
HEPES is a zwitterionic buffering agent commonly used in biological and biochemical applications to maintain stable pH conditions. It is particularly effective in the pH range of 6.8 to 8.2 and is widely used in cell culture, electrophoresis, and enzyme assays. Available in powder form with a molecular weight of 238.31 g/mol, CAS number 7365-45-9, and molecular formula C8H18N2O4S, HEPES is stable at ambient storage conditions. Molekula supplies HEPES from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Often bought together
Pricing & pack sizes
In stock — dispatched within 2 working days
These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.
Need a different size? Add the nearest pack and change the size in your basket — we price it at the same rate per unit.
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25 g — £30.50 per pack ex VAT · £36.60 inc. VAT
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100 g — £49.20 per pack ex VAT · £59.04 inc. VAT
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500 g — £88.80 per pack ex VAT · £106.56 inc. VAT
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1 kg — £153.20 per pack ex VAT · £183.84 inc. VAT
Need a different quantity or pack?
Tell us how much you need and when — drums, IBCs, sacks or a one-off bulk lot. We'll manufacture or source it and come back with a price.
Prices exclude VAT/sales tax and shipping.
Specifications
| Property | Value |
|---|---|
| Appearance | White free-flowing crystalline powder |
| Heavy metals | ≤5ppm |
| Identification | Conforms to reference |
| pH | 4.8 - 5.6 |
| Loss on Drying | ≤0.5% |
| Chloride (Cl-) | ≤100ppm |
| Assay | ≥99.5% |
| Residue on Ignition | ≤0.1% |
| Iron (Fe) | ≤5ppm |
| pKa 20°C | 7.35 - 7.69 |
| Solubility 10% in water | Clear, Colourless solution |
| A260 (1M aq) | ≤0.050 |
| A280 (1M water ) | ≤0.050 |
| Endotoxin (0.2% solution) | ≤0.1EU/ml |
| A405 (1M Water) | ≤0.015 |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | Ambient |
| Acidity | Acid |
Identification
| Property | Value |
|---|---|
| SKU | 47376574 |
| CAS Number | 7365-45-9 |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid |
| EC Number | 230-907-9 |
| Molecular Formula | C8H18N2O4S |
| Molecular Weight | 238.31 |
| Exact Mass | 238.0987 |
| SMILES | C1CN(CCN1CCO)CCS(=O)(=O)O |
| InChIKey | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| InChI | InChI=1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14) |
| PubChem CID | 23831 |
| REACH Code | Y113 |
| TSCA Listed | Yes |
| HTS Code | 2933.59.9500 |
| Tariff Code | 2933599590 |
Safety & GHS
Precautionary statements
- P280 Wear protective gloves/protective clothing/eye protection/face protection
Documents
Safety Data Sheets (SDS)
Available in English, English (US), French, German, Italian, Portuguese, Spanish.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Synonyms
- 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
- 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
- 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
- HEPES
- HEPES solution
- Hydroxyethyl Piperazine Ethanesulfonic Acid Solution
- hydroxyethylpiperazine ethane sulfonic acid
- Monosodium Salt, HEPES
- N 2 Hydroxyethylpiperazine N' 2' ethanesulfonic Acid
- Salt, HEPES Monosodium
Frequently asked questions
What is HEPES used for?
HEPES is commonly used as a buffering agent in biological and biochemical applications, particularly in cell culture and protein studies, due to its stability and effectiveness in the physiological pH range.
What is the chemical formula and molecular weight of HEPES?
The formula of HEPES is C8H18N2O4S with a molecular weight of 238.31 g/mol.
How should HEPES be stored?
HEPES should be stored at ambient temperature, protected from moisture and light, and kept in a tightly sealed container.
What grade of HEPES does Molekula supply?
Molekula supplies HEPES in high-purity grade, suitable for research and laboratory use.
Can Molekula supply HEPES in bulk?
Yes, Molekula can supply HEPES in bulk quantities—please contact our sales team for pricing and availability.
What documentation comes with a HEPES order?
Each order includes a Certificate of Analysis (CoA) and safety data sheet (SDS); additional documentation can be provided upon request.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.