Molekula
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid)
HEPES is a zwitterionic buffering agent commonly used in biological and biochemical applications to maintain stable pH conditions. It is particularly effective in the pH range of 6.8 to 8.2 and is widely used in cell culture, electrophoresis, and enzyme assays. Available in powder form with a molecular weight of 238.31 g/mol, CAS number 7365-45-9, and molecular formula C8H18N2O4S, HEPES is stable at ambient storage conditions. Molekula supplies HEPES from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Overview
HEPES is a zwitterionic buffering agent commonly used in biological and biochemical applications to maintain stable pH conditions. It is particularly effective in the pH range of 6.8 to 8.2 and is widely used in cell culture, electrophoresis, and enzyme assays. Available in powder form with a molecular weight of 238.31 g/mol, CAS number 7365-45-9, and molecular formula C8H18N2O4S, HEPES is stable at ambient storage conditions. Molekula supplies HEPES from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Specifications
| Property | Value |
|---|---|
| Appearance | White free-flowing crystalline powder |
| Heavy metals | ≤5ppm |
| Identification | Conforms to reference |
| pH | 4.8 - 5.6 |
| Loss on Drying | ≤0.5% |
| Chloride (Cl) | ≤100ppm |
| Assay | ≥99.5% |
| Residue on Ignition | ≤0.1% |
| Iron (Fe) | ≤5ppm |
| pKa (@ 20°C) | 7.35 - 7.69 |
| A260 (1M water ) | ≤0.050 |
| A280 (1M water ) | ≤0.050 |
| Endotoxin ( 0.2% solution ) | ≤0.1EU/ml |
| A405 (1M Water) | ≤0.015 |
| Solubility 1M water | Clear, Colourless solution |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | Ambient |
| Shelf Life | 24 months |
| Acidity | Acid |
Identification
| Property | Value |
|---|---|
| CAS Number | 7365-45-9 |
| Molecular Formula | C8H18N2O4S |
| Molecular Weight | 238.31 |
| SMILES | C1CN(CCN1CCO)CCS(=O)(=O)O |
| REACH Code | Y113 |
| TSCA Listed | Yes |
| HTS Code | 2933.59.9500 |
| Tariff Code | 2933599590 |
Safety & GHS
Precautionary statements
- P280 Wear protective gloves/protective clothing/eye protection/face protection
For research and industrial use only
Documents
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Pricing & pack sizes
Synonyms
No alternate names recorded for this product.
Frequently asked questions
What is HEPES used for?
HEPES is commonly used as a buffering agent in biological and biochemical applications, particularly in cell culture and protein studies, due to its stability and effectiveness in the physiological pH range.
What is the chemical formula and molecular weight of HEPES?
The formula of HEPES is C8H18N2O4S with a molecular weight of 238.31 g/mol.
How should HEPES be stored?
HEPES should be stored at ambient temperature, protected from moisture and light, and kept in a tightly sealed container.
What grade of HEPES does Molekula supply?
Molekula supplies HEPES in high-purity grade, suitable for research and laboratory use.
Can Molekula supply HEPES in bulk?
Yes, Molekula can supply HEPES in bulk quantities—please contact our sales team for pricing and availability.
What documentation comes with a HEPES order?
Each order includes a Certificate of Analysis (CoA) and safety data sheet (SDS); additional documentation can be provided upon request.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.