Molekula
3,3,5,5-Tetramethylbenzidine
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 2000 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
3,3,5,5-Tetramethylbenzidine is a sensitive chromogenic substrate used in enzyme-linked immunosorbent assays (ELISAs) and other immunochemical detection methods. It produces a blue color upon oxidation, which can be measured spectrophotometrically. It also serves as a clinical laboratory reagent in tests for detecting blood in samples. CAS: 54827-17-7, Molecular Formula: C16H20N2, Molecular Weight: 240.34 g/mol. Store at 2 to 8 °C. Signal word: Danger. Molekula supplies 3,3,5,5-Tetramethylbenzidine from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Pricing & pack sizes
These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.
Need a different size? Add the nearest pack and change the size in your basket — we price it at the same rate per unit.
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5 g — $209.00 per pack
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25 g — $590.90 per pack
Need a different quantity or pack?
Tell us how much you need and when — drums, IBCs, sacks or a one-off bulk lot. We'll manufacture or source it and come back with a price.
Prices exclude VAT/sales tax and shipping.
Specifications
| Property | Value |
|---|---|
| Appearance | White, off white to grey or yellow powder |
| Identification | Conforms to reference |
| Loss on Drying | ≤2.0% |
| Purity | ≥99% |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | 2 to 8 °C |
| Transport Class | 9 |
| Packing Group | III |
| Acidity | Base |
For research use only. Not for human use.
Literature values
Published physical properties of the substance, not batch specifications.
| Property | Value |
|---|---|
| Melting Point | 168.5 °C |
| Vapour Pressure | 0.0000005 mmHg |
Source: PubChem CID 41206 (HSDB, Haz-Map)
Identification
| Property | Value |
|---|---|
| SKU | 29622681 |
| CAS Number | 54827-17-7 |
| IUPAC Name | 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline |
| EC Number | 259-364-6 |
| Molecular Formula | C16H20N2 |
| Molecular Weight | 240.34 |
| Exact Mass | 240.1626 |
| SMILES | CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C |
| InChIKey | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| InChI | InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| PubChem CID | 41206 |
| UN Number | UN3077 |
| REACH Code | Y110 |
| TSCA Listed | Yes |
| HTS Code | 29215980 |
| Tariff Code | 2921599090 |
Safety & GHS
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Health Hazard
GHS07
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Serious Health Hazard
GHS08
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Hazardous to the Environment
GHS09
Hazard statements
- H302 Harmful if swallowed
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H341 Suspected of causing genetic defects
- H351 Suspected of causing cancer
- H410 Very toxic to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P302+352 IF ON SKIN: Wash with soap and water
- P305+351+338 IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing
- P308+313 IF exposed or concerned: Get medical advice/attention
Documents
Safety Data Sheets (SDS)
Available in English, French, German, Italian, Portuguese, Spanish.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Synonyms
- (1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-
- 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine
- 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine
- 3,5,3',5'-Tetramethylbenzidine
- 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
- 4,4'-Bi-2,6-xylidine
- COLORBURST BLUE
- POTASSIUM-BLUE MAX
- tetramethyl benzidine
- TMB
Frequently asked questions
What is 3,3,5,5-Tetramethylbenzidine used for?
It is primarily used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISAs) for detecting antigens or antibodies.
What is the chemical formula and molecular weight of 3,3,5,5-Tetramethylbenzidine?
The formula is C16H20N2 with a molecular weight of 240.34 g/mol.
How should 3,3,5,5-Tetramethylbenzidine be stored?
It should be stored at 2 to 8 °C to maintain stability and prevent degradation.
What grade is available for this product?
It is available in high-purity research grade suitable for laboratory use.
Can Molekula supply 3,3,5,5-Tetramethylbenzidine in bulk?
Yes, Molekula can supply this compound in bulk quantities—please contact our sales team for pricing and availability.
What documentation comes with an order of 3,3,5,5-Tetramethylbenzidine?
Each order includes a Certificate of Analysis (CoA) and Safety Data Sheet (SDS) with the shipment.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.