Molekula
3,3,5,5-Tetramethylbenzidine
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 1999 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
3,3,5,5-Tetramethylbenzidine is a sensitive chromogenic substrate used in enzyme-linked immunosorbent assays (ELISAs) and other immunochemical detection methods. It produces a blue color upon oxidation, which can be measured spectrophotometrically. It also serves as a clinical laboratory reagent in tests for detecting blood in samples. CAS: 54827-17-7, Molecular Formula: C16H20N2, Molecular Weight: 240.34 g/mol. Store at 2 to 8 °C. Signal word: Danger. Molekula supplies 3,3,5,5-Tetramethylbenzidine from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Pricing & pack sizes
In stock — same-day dispatch
These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.
Need a different size? Add the nearest pack and change the size in your basket — we price it at the same rate per unit.
-
5 g — £110.00 per pack ex VAT · £132.00 inc. VAT
-
25 g — £311.00 per pack ex VAT · £373.20 inc. VAT
Need a different quantity or pack?
Tell us how much you need and when — drums, IBCs, sacks or a one-off bulk lot. We'll manufacture or source it and come back with a price.
Prices exclude VAT/sales tax and shipping.
Specifications
| Property | Value |
|---|---|
| Appearance | White, off white to grey or yellow powder |
| Identification | Conforms to reference |
| Loss on Drying | ≤2.0% |
| Purity | ≥99% |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | 2 to 8 °C |
| Transport Class | 9 |
| Packing Group | III |
| Acidity | Base |
For research use only. Not for human use.
Literature values
Published physical properties of the substance, not batch specifications.
| Property | Value |
|---|---|
| Melting Point | 168.5 °C |
| Vapour Pressure | 0.0000005 mmHg |
Source: PubChem CID 41206 (HSDB, Haz-Map)
Identification
| Property | Value |
|---|---|
| SKU | 29622681 |
| CAS Number | 54827-17-7 |
| IUPAC Name | 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline |
| EC Number | 259-364-6 |
| Molecular Formula | C16H20N2 |
| Molecular Weight | 240.34 |
| Exact Mass | 240.1626 |
| SMILES | CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C |
| InChIKey | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| InChI | InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| PubChem CID | 41206 |
| UN Number | UN3077 |
| REACH Code | Y110 |
| TSCA Listed | Yes |
| HTS Code | 29215980 |
| Tariff Code | 2921599090 |
Safety & GHS
-
Health Hazard
GHS07
-
Serious Health Hazard
GHS08
-
Hazardous to the Environment
GHS09
Hazard statements
- H302 Harmful if swallowed
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
- H341 Suspected of causing genetic defects
- H351 Suspected of causing cancer
- H410 Very toxic to aquatic life with long lasting effects
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P273 Avoid release to the environment
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P302+352 IF ON SKIN: Wash with soap and water
- P305+351+338 IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing
- P308+313 IF exposed or concerned: Get medical advice/attention
Documents
Safety Data Sheets (SDS)
Available in English, French, German, Italian, Portuguese, Spanish.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Quality certificates
Molekula Group is certified to ISO 9001:2015 (quality) and ISO 14001:2015 (environmental management), audited by a UKAS-accredited body. Both certificates are valid to 17 May 2028.
Synonyms
- (1,1'-Biphenyl)-4,4'-diamine, 3,3',5,5'-tetramethyl-
- 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine
- 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine
- 3,5,3',5'-Tetramethylbenzidine
- 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
- 4,4'-Bi-2,6-xylidine
- COLORBURST BLUE
- POTASSIUM-BLUE MAX
- tetramethyl benzidine
- TMB
Frequently asked questions
What is 3,3,5,5-Tetramethylbenzidine used for?
It is primarily used as a chromogenic substrate in enzyme-linked immunosorbent assays (ELISAs) for detecting antigens or antibodies.
What is the chemical formula and molecular weight of 3,3,5,5-Tetramethylbenzidine?
The formula is C16H20N2 with a molecular weight of 240.34 g/mol.
How should 3,3,5,5-Tetramethylbenzidine be stored?
It should be stored at 2 to 8 °C to maintain stability and prevent degradation.
What grade is available for this product?
It is available in high-purity research grade suitable for laboratory use.
Can Molekula supply 3,3,5,5-Tetramethylbenzidine in bulk?
Yes, Molekula can supply this compound in bulk quantities—please contact our sales team for pricing and availability.
What documentation comes with an order of 3,3,5,5-Tetramethylbenzidine?
Each order includes a Certificate of Analysis (CoA) and Safety Data Sheet (SDS) with the shipment.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.