Molekula
2',7'-Dichlorodihydrofluorescein diacetate
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 2000 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is a non-fluorescent cell-permeable compound widely used as a probe to detect reactive oxygen species (ROS) and oxidative stress in biological research. It is hydrolyzed by esterases to form DCFH, which is then oxidized to the highly fluorescent 2',7'-dichlorofluorescein (DCF) in the presence of ROS. This chemical has a CAS number of 4091-99-0, a molecular formula of C24H16Cl2O7, and a molecular weight of 487.29 g/mol. It should be stored at -15 to -20°C. The signal word for this product is "Warning." Molekula supplies 2',7'-Dichlorodihydrofluorescein diacetate from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Pricing & pack sizes
These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.
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50 mg — £65.00 per pack ex VAT · £78.00 inc. VAT
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Specifications
| Property | Value |
|---|---|
| Appearance | White to pale yellow powder |
| Purity (HPLC) | ≥97.0% |
| Purity (TLC) | ≥97.0% |
| Water (KF) | ≥0.00% |
| Solubility (Turbidity) | Clear |
| Solubility (Colour) | Colourless or faint yellow |
| Identification (NMR) | Consistent |
| solubility (heating) | Yes or no |
| Solubility (conc) | 9.80 - 10.20 mg/ml |
| Solubility (solvent) | Methanol |
| Elemental analysis (%C anhydrous) | 58.65 - 59.65% |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | -15 to -20 °C |
| Acidity | Neutral |
Identification
| Property | Value |
|---|---|
| SKU | 90034702 |
| CAS Number | 4091-99-0 |
| IUPAC Name | 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid |
| Molecular Formula | C24H16Cl2O7 |
| Molecular Weight | 487.29 |
| Exact Mass | 486.0273 |
| SMILES | CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl |
| InChIKey | PXEZTIWVRVSYOK-UHFFFAOYSA-N |
| InChI | InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30) |
| PubChem CID | 77718 |
| REACH Code | Y113 |
| TSCA Listed | Yes |
| HTS Code | 2932997000 |
| Tariff Code | 2932990090 |
Safety & GHS
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Health Hazard
GHS07
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
Precautionary statements
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P302+352 IF ON SKIN: Wash with soap and water
- P305+351+338 IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing
- P333+313 IF skin irritation or a rash occurs: Get medical advice/attention
- P337+313 If eye irritation persists get medical advice/attention
Documents
Safety Data Sheets (SDS)
Available in English, French, German, Italian, Spanish.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Synonyms
- 2-(3,6-diacetoxy-2,7-dichloro-9h-xanthen-9-yl)benzoic acid
- 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
- 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic Acid
- 2',7'-Dichlorohydrofluorescein diacetate
- Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
- C24H16Cl2O7
- DCDHF-DA
- H2DCFDA
- H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate)
- H2DCFDA(DCFH-DA)
Frequently asked questions
What is 2',7'-Dichlorodihydrofluorescein diacetate (CAS 4091-99-0) used for?
It is commonly used as a fluorescent probe for detecting reactive oxygen species (ROS) and hydrogen peroxide in biological research.
What is the chemical formula and molecular weight?
The formula is **C₂₄H₁₆Cl₂O₇** with a molecular weight of **487.29 g/mol**.
What safety classification does this compound have?
The product is labeled with the **signal word 'Warning'** under GHS classification.
How should this compound be stored?
Store at **-15 to -20°C** to maintain stability and prevent degradation.
Does Molekula supply this product in bulk quantities?
Yes, we can supply this compound in bulk—please contact us for pricing and lead times.
What documentation is provided with an order of this product?
Each order includes a **Certificate of Analysis (CoA)**, **Material Safety Data Sheet (MSDS/SDS)**, and a **batch-specific quality report**.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.