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Molekula

Molekula

2',7'-Dichlorodihydrofluorescein diacetate

SKU 90034702 CAS 4091-99-0 C24H16Cl2O7 MW 487.29 Made to order Bulk quantities on request

Supplying 4,500+ customers in over 80 countries

ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 1999 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need

2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is a non-fluorescent cell-permeable compound widely used as a probe to detect reactive oxygen species (ROS) and oxidative stress in biological research. It is hydrolyzed by esterases to form DCFH, which is then oxidized to the highly fluorescent 2',7'-dichlorofluorescein (DCF) in the presence of ROS. This chemical has a CAS number of 4091-99-0, a molecular formula of C24H16Cl2O7, and a molecular weight of 487.29 g/mol. It should be stored at -15 to -20°C. The signal word for this product is "Warning."

Molekula supplies 2',7'-Dichlorodihydrofluorescein diacetate at ≥97.0% purity (HPLC) in 50 mg packs, from £78.00 inc. VAT. It is made or sourced to order. Every batch ships with a certificate of analysis, and safety data sheets are available in 5 languages. Larger quantities, up to multi-tonne, are quoted on request.

Expect a response within hours Speak to a chemist: +44 (0) 3302 000333

Example image for illustration only. Other packaging and pack sizes are available.

Pricing & pack sizes

Pack sizes & pricing

On backorder — expected to ship by 5 December 2026

These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.

Need a different size? Add the nearest pack and change the size in your basket — we price it at the same rate per unit.

  • 50 mg — £65.00 per pack ex VAT · £78.00 inc. VAT

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Specifications

Property Value
Appearance White to pale yellow powder
Purity (HPLC) ≥97.0%
Purity (TLC) ≥97.0%
Water (KF) ≥0.00%
Solubility (Turbidity) Clear
Solubility (Colour) Colourless or faint yellow
Identification (NMR) Consistent
solubility (heating) Yes or no
Solubility (conc) 9.80 - 10.20 mg/ml
Solubility (solvent) Methanol
Elemental analysis (%C anhydrous) 58.65 - 59.65%

Storage & handling

Property Value
Storage Temperature -15 to -20 °C
Acidity Neutral

Identification

Property Value
SKU 90034702
CAS Number 4091-99-0
IUPAC Name 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
Molecular Formula C24H16Cl2O7
Molecular Weight 487.29
Exact Mass 486.0273
SMILES CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)O)Cl
InChIKey PXEZTIWVRVSYOK-UHFFFAOYSA-N
InChI InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
PubChem CID 77718
REACH Code Y113
TSCA Listed Yes
HTS Code 2932997000
Tariff Code 2932990090

Safety & GHS

Warning
  • GHS pictogram — Health Hazard Health Hazard GHS07

Hazard statements

  • H315 Causes skin irritation
  • H319 Causes serious eye irritation

Precautionary statements

  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P302+352 IF ON SKIN: Wash with soap and water
  • P305+351+338 IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing
  • P333+313 IF skin irritation or a rash occurs: Get medical advice/attention
  • P337+313 If eye irritation persists get medical advice/attention

Documents

Safety Data Sheets (SDS)

Available in English, French, German, Italian, Spanish.

Certificate of Analysis (CoA) by batch

Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.

Quality certificates

Molekula Group is certified to ISO 9001:2015 (quality) and ISO 14001:2015 (environmental management), audited by a UKAS-accredited body. Both certificates are valid to 17 May 2028.

Synonyms

  • 2-(3,6-diacetoxy-2,7-dichloro-9h-xanthen-9-yl)benzoic acid
  • 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid
  • 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic Acid
  • 2',7'-Dichlorohydrofluorescein diacetate
  • Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]-
  • C24H16Cl2O7
  • DCDHF-DA
  • H2DCFDA
  • H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate)
  • H2DCFDA(DCFH-DA)

Frequently asked questions

What is 2',7'-Dichlorodihydrofluorescein diacetate (CAS 4091-99-0) used for?

It is commonly used as a fluorescent probe for detecting reactive oxygen species (ROS) and hydrogen peroxide in biological research.

What is the chemical formula and molecular weight?

The formula is C₂₄H₁₆Cl₂O₇ with a molecular weight of 487.29 g/mol.

What safety classification does this compound have?

The product is labeled with the signal word 'Warning' under GHS classification.

How should this compound be stored?

Store at -15 to -20°C to maintain stability and prevent degradation.

Does Molekula supply this product in bulk quantities?

Yes, we can supply this compound in bulk—please contact us for pricing and lead times.

What documentation is provided with an order of this product?

Each order includes a Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS/SDS), and a batch-specific quality report.

Where can I buy 2',7'-Dichlorodihydrofluorescein diacetate?

Directly from Molekula, which lists 2',7'-Dichlorodihydrofluorescein diacetate in 50 mg packs from £78.00 inc. VAT, with a certificate of analysis for every batch. Larger quantities are quoted on request. Molekula's offices: United Kingdom, France, Spain, Germany, Italy, United States and China.

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