Molekula
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 1999 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate is an organic compound primarily used as a chromogenic substrate in biochemical assays, particularly in ELISA and Western blotting for detecting peroxidase activity. It reacts with hydrogen peroxide to produce a blue precipitate, enabling sensitive detection of target proteins. CAS number 207738-08-7, molecular formula C16H20N2·2HCl·xH2O, molecular weight 313.27. Store between 2–8°C. Signal word: Warning. Molekula supplies 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Pricing & pack sizes
Pack sizes & pricing
Pricing on request — packs available from research to bulk quantities.
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Specifications
| Property | Value |
|---|---|
| Appearance | White to almost white light fluffy crystalline powder |
| Loss on Drying | ≤11.0% |
| Assay | ≥99.0% |
| Residue on Ignition | ≤0.1% |
| Solubility (1M in H2O) | Clear, colourless solution |
| UV Absorption (280nm) | (10mg/L aq.) ≤1.0% |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | 2 to 8 °C |
| Transport Class | 6.1 |
| Packing Group | III |
| Acidity | Neutral |
Identification
| Property | Value |
|---|---|
| SKU | 90030326 |
| CAS Number | 207738-08-7 |
| IUPAC Name | 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline;hydrate;dihydrochloride |
| Molecular Formula | C16H20N2 . 2 HCl . xH2 O |
| Molecular Weight | 313.27 |
| Exact Mass | 330.1266 |
| SMILES | CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C.O.Cl.Cl |
| InChIKey | KVCWTKDFVVSVSJ-UHFFFAOYSA-N |
| InChI | InChI=1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2 |
| PubChem CID | 19083738 |
| UN Number | UN2811 |
| REACH Code | Y110 |
| HTS Code | 2921598090 |
| Tariff Code | 2921599090 |
Safety & GHS
-
Health Hazard
GHS07
-
Serious Health Hazard
GHS08
Hazard statements
- H302 Harmful if swallowed
- H341 Suspected of causing genetic defects
- H350 May cause cancer
Precautionary statements
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P263 Avoid contact during pregnancy/while nursing
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P301+310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician
- P302+352 IF ON SKIN: Wash with soap and water
- P304+340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
Documents
Safety Data Sheets (SDS)
Available in English, French, German, Italian, Portuguese, Spanish.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Synonyms
- 3,3',5,5'-TETRAMETHYL-[1,1'-BIPHENYL]-4,4'-DIAMINE 2HCL HYDRATE
- 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride hydrate
- 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine dihydrochloride xhydrate
- 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate
- 3,3',5,5'-Tetramethylbenzidine dihydrochloride dihydrate
- 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline;hydrate;dihydrochloride
- KVCWTKDFVVSVSJ-UHFFFAOYSA-N
- TMB (hydrochloride hydrate)
Frequently asked questions
What is 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate commonly used for?
This compound is primarily used as a chromogenic substrate in immunoassays, such as ELISA, and in biochemical research for detection applications.
What is the chemical formula and molecular weight of this product?
The formula is C₁₆H₂₀N₂ · 2 HCl · xH₂O, and the molecular weight is 313.27 g/mol (anhydrous dihydrochloride salt, C₁₆H₂₀N₂ · 2 HCl). The free base, C₁₆H₂₀N₂, has a molecular weight of about 240.35 g/mol.
How should this product be stored?
Store at 2–8°C in a cool, dry place, away from direct light and moisture. Follow local regulations for hazardous chemical storage.
What grade or purity is available for this compound?
Molekula supplies this product in analytical/biochemical grade, suitable for research and diagnostic applications.
Can Molekula supply this product in bulk quantities?
Yes, we can provide bulk quantities upon request—please contact our sales team for pricing and availability.
What documentation comes with an order of this product?
Each order includes a Certificate of Analysis (CoA), Material Safety Data Sheet (MSDS), and a product specification sheet.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.