Molekula
2,7-Dichlorofluorescein
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 2000 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
2,7-Dichlorofluorescein is a synthetic fluorescent dye widely used in biological research, flow cytometry, and forensic analysis as a pH indicator and tracer. It fluoresces under UV light, making it useful as an adsorption indicator in argentometric (Fajans) titrations, a fluorescent pH indicator, a visualization reagent in thin-layer chromatography, and the fluorescent product read out in DCFH-DA reactive oxygen species assays. This high-purity compound has a CAS number of 76-54-0, a molecular formula of C20H10Cl2O5, and a molecular weight of 401.20 g/mol. It should be stored at ambient conditions and carries a signal word of "Warning" for safety. Molekula supplies 2,7-Dichlorofluorescein from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Pricing & pack sizes
These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.
Need a different size? Add the nearest pack and change the size in your basket — we price it at the same rate per unit.
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5 g — $88.70 per pack
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Specifications
| Property | Value |
|---|---|
| Appearance | Crystalline solid |
| Purity | ⩾ 95.0% |
| Clarity in Solution | Passes test |
| Water (KF) | ≤ 0.5% |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | Ambient |
| Flash Point | 0 °C |
| Transport Class | Not regulated |
| Acidity | Acid |
Identification
| Property | Value |
|---|---|
| SKU | 20935505 |
| CAS Number | 76-54-0 |
| IUPAC Name | 2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one |
| EC Number | 200-968-6 |
| Molecular Formula | C20H10Cl2O5 |
| Molecular Weight | 401.20 |
| Exact Mass | 399.9905 |
| SMILES | C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl |
| InChIKey | VFNKZQNIXUFLBC-UHFFFAOYSA-N |
| InChI | InChI=1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H |
| PubChem CID | 64944 |
| UN Number | UN1 |
| REACH Code | Y113 |
| TSCA Listed | Yes |
| HTS Code | 3212.90.0050 |
| Tariff Code | 3212900000 |
Safety & GHS
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Health Hazard
GHS07
Hazard statements
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P302+352 IF ON SKIN: Wash with soap and water
- P305+P351+P338 IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do, continue rinsing
Documents
Safety Data Sheets (SDS)
Available in English, German, Spanish, French, Italian, Portuguese, Arabic, Dutch.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Synonyms
- 11,16-dichloro-12,15-dihydroxyspiro[3-hydroisobenzofuran-3,9'-xanthene]-1-one
- 2',7'-bis(chloranyl)-3',6'-bis(oxidanyl)spiro[2-benzofuran-3,9'-xanthene]-1-one
- 2',7'-Dichloro-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
- 2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
- 2',7'-Dichlorofluorescein [Fluorescent indicator]
- 2',7'-dichlorofluorescin
- D AND C ORANGE 25
- Fluorescein 27
- FLUORESCEIN 548
- Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 2',7'-dichloro-3',6'-dihydroxy-
Frequently asked questions
What is 2,7-Dichlorofluorescein commonly used for?
2,7-Dichlorofluorescein is primarily used as a pH indicator (fluorescein derivative) in analytical chemistry and as a fluorescent dye in biological staining, microscopy, and forensic applications.
What is the chemical formula and molecular weight of 2,7-Dichlorofluorescein?
The chemical formula is **C₂₀H₁₀Cl₂O₅**, and the molecular weight is **401.20 g/mol**.
How should 2,7-Dichlorofluorescein be stored?
Store in a tightly sealed container at **ambient temperature**, protected from light and moisture to prevent degradation.
What purity grades are available for this product?
Molekula supplies 2,7-Dichlorofluorescein in **analytical reagent (AR) grade** and **biological/fluorescent grade** (98%+ purity).
Can Molekula supply 2,7-Dichlorofluorescein in bulk quantities?
Yes, we offer bulk packaging options (e.g., 5 kg, 25 kg, and custom quantities) for industrial or large-scale applications.
What documentation is provided with an order of 2,7-Dichlorofluorescein?
Each order includes a **Certificate of Analysis (CoA)**, **Material Safety Data Sheet (MSDS)**, and a **batch-specific label** with hazard warnings (Signal Word: *Warning*).
Ready to order or need a custom pack?
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