Molekula
5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside (X-alpha-gal)
Supplying 4,500+ customers in over 80 countries
ISO 9001:2015 & ISO 14001:2015 certified · Supplying laboratories and industry since 1999 · Listed pack sizes are a starting point — we manufacture, source and repack to the quantity you need
5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside (X-alpha-gal) is a chromogenic substrate used in enzymatic assays, particularly for detecting alpha-galactosidase activity, such as expression of the MEL1 reporter gene in yeast two-hybrid systems. It is a key reagent in molecular biology and histology applications. CAS: 107021-38-5, Molecular Formula: C14H15BrClNO6, Molecular Weight: 408.63 g/mol. Stored at -15 to -20 °C. Molekula supplies X-alpha-gal from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Example image for illustration only. Other packaging and pack sizes are available.
Pricing & pack sizes
On backorder — expected to ship by 24 November 2026
These pack sizes aren't a limit. Molekula manufactures, sources and repacks to order — if you need 500 kg and only see 1 kg listed, . We quote from grams to multi-tonne.
Need a different size? Add the nearest pack and change the size in your basket — we price it at the same rate per unit.
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100 mg — £30.90 per pack ex VAT · £37.08 inc. VAT
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250 mg — £87.10 per pack ex VAT · £104.52 inc. VAT
Need a different quantity or pack?
Tell us how much you need and when — drums, IBCs, sacks or a one-off bulk lot. We'll manufacture or source it and come back with a price.
Prices exclude VAT/sales tax and shipping.
Specifications
| Property | Value |
|---|---|
| Appearance | White to off-white powder |
| Water | ≤2% |
| Specific optical rotation | +130 to +148 |
| Purity (TLC) | ≥99% |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | -15 to -20 °C |
Identification
| Property | Value |
|---|---|
| SKU | 87527781 |
| CAS Number | 107021-38-5 |
| IUPAC Name | (2R,3R,4S,5R,6R)-2-[(5-bromo-4-chloro-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.63 |
| Exact Mass | 406.9771 |
| SMILES | C1=CC(=C(C2=C1NC=C2O[C@@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br |
| InChIKey | OPIFSICVWOWJMJ-YGEXGZRRSA-N |
| InChI | InChI=1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14+/m1/s1 |
| PubChem CID | 10173103 |
| REACH Code | Y110 |
| HTS Code | 29400060 |
| Tariff Code | 2940000080 |
Safety & GHS
Precautionary statements
- P280 Wear protective gloves/protective clothing/eye protection/face protection
Documents
Safety Data Sheets (SDS)
Available in English, French, German, Italian, Spanish.
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Quality certificates
Molekula Group is certified to ISO 9001:2015 (quality) and ISO 14001:2015 (environmental management), audited by a UKAS-accredited body. Both certificates are valid to 17 May 2028.
Synonyms
- 5-Bromo-4-chloro-3-indolyl a-D-galactopyranoside
- 5-Bromo-4-chloro-3-indolyl a-D-glucopyranoside
- 5-Bromo-4-chloro-3-indolyl alpha-D-galactopyranoside
- 5-bromo-4-chloro-3-indolyl alpha-D-galactoside
- 5-bromo-4-chloro-3-indolyl--d-galactopyranoside
- 5-Bromo-4-chloro-3-indoxyl-alpha-D-galactopyranoside
- 5-bromo-4-chloroindoxyl alpha-D-galactoside
- X-?-Gal
- X-| cent-D-Gal
- X-alpha-Gal
Frequently asked questions
What is 5-Bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside (X-alpha-gal) used for?
It is primarily used as a chromogenic substrate for detecting alpha-galactosidase enzyme activity in molecular biology applications, such as detecting MEL1 reporter gene expression in yeast two-hybrid assays.
What is the chemical formula and molecular weight of X-alpha-gal?
The formula is C14H15BrClNO6 with a molecular weight of 408.63 g/mol.
How should X-alpha-gal be stored?
It should be stored at temperatures between -15 and -20 °C to maintain stability and prevent degradation.
What grade is available for X-alpha-gal?
Available in high-purity research grade suitable for laboratory use in enzymatic assays and molecular biology.
Can Molekula supply X-alpha-gal in bulk?
Yes, Molekula can supply X-alpha-gal in bulk quantities; please contact our sales team for pricing and availability.
What documentation comes with an order of X-alpha-gal?
Each order includes a Certificate of Analysis (CoA) and safety data sheet (SDS); additional documentation can be provided upon request.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.