Application
Electrophoresis & blotting
Running buffers, stains, and transfer reagents for SDS-PAGE, native PAGE, and Western blotting.
Protein and nucleic-acid electrophoresis runs in a Tris-glycine or Tris-acetate buffer, separates by size in a polyacrylamide or agarose gel, and is visualised with Coomassie, silver, or fluorescent stain. Molekula supplies electrophoresis-grade Tris, glycine, SDS, acrylamide, TEMED, and APS.
What's in this workflow
- Running buffers
- Tris-glycine (SDS-PAGE), Tris-tricine (low-MW peptides), TAE/TBE (agarose nucleic acid).
- Gel monomers
- Acrylamide, bis-acrylamide, TEMED, ammonium persulfate (APS).
- Detergents & denaturants
- SDS, urea.
- Stains
- Coomassie Brilliant Blue R-250 / G-250, silver nitrate.
- Transfer
- Methanol-Tris-glycine, semi-dry / wet-tank.
Products from our catalogue
Live matches from the Molekula catalogue — request a quote on any product page.
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FAQ
What percentage acrylamide gel should I use?
12% is a good default for 10–80 kDa proteins; 8% resolves larger proteins; 15% for small peptides. Use Tris-tricine for proteins below 20 kDa.
How is APS used in PAGE gels?
APS (ammonium persulfate) is the radical initiator; TEMED accelerates the polymerization. Standard recipe is 0.05% APS + 0.05% TEMED in the gel mix.
Need a custom grade or pack size?
Talk to a chemist — we run process chemistry from grams up to multi-kilogram campaigns.
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