Molekula
X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside)
X-Gal is a chromogenic substrate used in molecular biology for detecting beta-galactosidase enzyme activity, commonly in blue-white screening of recombinant DNA clones. It is a white to off-white crystalline powder with a molecular formula of C14H15BrClNO6 and a molecular weight of 408.63 g/mol. It is stored at -15 to -20 °C and carries a warning signal word due to potential hazards. Molekula supplies X-Gal from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Overview
X-Gal is a chromogenic substrate used in molecular biology for detecting beta-galactosidase enzyme activity, commonly in blue-white screening of recombinant DNA clones. It is a white to off-white crystalline powder with a molecular formula of C14H15BrClNO6 and a molecular weight of 408.63 g/mol. It is stored at -15 to -20 °C and carries a warning signal word due to potential hazards. Molekula supplies X-Gal from research to bulk quantities with full documentation and ISO 9001:2015 traceability.
Specifications
| Property | Value |
|---|---|
| Appearance | White to off-white crystalline powder |
| Appearance of Solution | Clear, colourless to light yellow solution (50mg/ml in DMF:MeOH, 1:1) |
| Specific optical rotation | -61.5 +/- 1 |
| Purity HPLC | min 99% |
| Solubility (5 % in DMF) | Soluble (5% w/v,DMF) |
| Water KF | max 1% |
| Assay (HPLC on Anhydrous Basis) | min 98% w/w |
Storage & handling
| Property | Value |
|---|---|
| Storage Temperature | -15 to -20 °C |
| Shelf Life | 24 months |
| Acidity | Neutral |
Identification
| Property | Value |
|---|---|
| CAS Number | 7240-90-6 |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Weight | 408.63 |
| SMILES | C1=CC(=C(C2=C1NC=C2OC3C(C(C(C(O3)CO)O)O)O)Cl)Br |
| REACH Code | Y110 |
| HTS Code | 29400060 |
| Tariff Code | 2940000080 |
Safety & GHS
-
Explosive
GHS01
Hazard statements
- H302 + H312 + H332 Harmful if swallowed, in contact with skin or if inhaled
Precautionary statements
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P301+312 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell
- P302+352 IF ON SKIN: Wash with soap and water
- P304+340 IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
- P305+351+338 IF IN EYES: Rinse with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing
For research and industrial use only
Documents
Certificate of Analysis (CoA) by batch
Enter a lot or batch number to retrieve the CoA for that specific batch. We can generate one from spec data if no PDF is on file.
Pricing & pack sizes
Synonyms
No alternate names recorded for this product.
Frequently asked questions
What is X-Gal used for?
X-Gal is used as a chromogenic substrate for beta-galactosidase in molecular biology, commonly in blue-white screening of recombinant DNA clones.
What is the chemical formula and molecular weight of X-Gal?
The formula is C14H15BrClNO6 with a molecular weight of 408.63 g/mol.
How should X-Gal be stored?
X-Gal should be stored at -15 to -20 °C to maintain stability and prevent degradation.
What grade of X-Gal is available?
Molekula supplies X-Gal in high-purity grade suitable for research use.
Can Molekula supply X-Gal in bulk?
Yes, Molekula can supply X-Gal in bulk quantities—please contact sales for pricing and availability.
What documentation comes with an X-Gal order?
Each order includes a Certificate of Analysis and Safety Data Sheet (SDS) with the product.
Ready to order or need a custom pack?
Tell us the product, grade, and quantity — Molekula usually responds within one working day with pricing and lead time.