Anwendung
PCR & qPCR reagents
Bulk-quality Tris, MgCl₂, KCl and nucleotides for nucleic-acid amplification.
PCR reagents are the buffer salts and cofactors that make DNA polymerases work: Tris-HCl (pH 8.3–8.8) for the reaction buffer, KCl for ionic strength, MgCl₂ as a polymerase cofactor, dNTPs for chain extension, and EDTA for stopping reactions. Molekula supplies molecular-biology-grade and ACS-grade salts with full Certificates of Analysis per batch.
Was enthält dieses Workflow
- Reaction buffer
- Tris-HCl, KCl, (NH₄)₂SO₄ — the salts that keep polymerase active and Mg²⁺ in solution.
- Cofactors
- MgCl₂ (1.5–4 mM typical), MgSO₄ for Pfu, MnCl₂ for low-fidelity readout.
- Templates
- dNTPs in molecular-biology grade.
- Stops & cleanup
- EDTA, ammonium acetate, NaOAc.
Produkte aus unserem Katalog
Aktuelle Angebote aus dem Molekula-Katalog — rufen Sie ein Angebot auf jeder Produktdetailseite an.
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Häufig gestellte Fragen
What grade of Tris should I use for PCR?
Molecular-biology grade (DNase/RNase-free) is recommended. ACS-grade also works for routine PCR but lacks the certified nuclease-free testing.
What MgCl₂ concentration is best?
1.5–2.0 mM is the standard starting point. Optimize between 1 and 4 mM for tricky templates — more Mg²⁺ relaxes specificity, less Mg²⁺ tightens it.
Does Molekula supply DNase/RNase-free buffer salts?
Yes — many products are available as molecular-biology grade with nuclease testing on the CoA. Request the molecular-biology grade when quoting.
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