Application
PCR & qPCR reagents
Bulk-quality Tris, MgCl₂, KCl and nucleotides for nucleic-acid amplification.
PCR reagents are the buffer salts and cofactors that make DNA polymerases work: Tris-HCl (pH 8.3–8.8) for the reaction buffer, KCl for ionic strength, MgCl₂ as a polymerase cofactor, dNTPs for chain extension, and EDTA for stopping reactions. Molekula supplies molecular-biology-grade and ACS-grade salts with full Certificates of Analysis per batch.
What's in this workflow
- Reaction buffer
- Tris-HCl, KCl, (NH₄)₂SO₄ — the salts that keep polymerase active and Mg²⁺ in solution.
- Cofactors
- MgCl₂ (1.5–4 mM typical), MgSO₄ for Pfu, MnCl₂ for low-fidelity readout.
- Templates
- dNTPs in molecular-biology grade.
- Stops & cleanup
- EDTA, ammonium acetate, NaOAc.
Products from our catalogue
Live matches from the Molekula catalogue — request a quote on any product page.
Related products
-
EDTA dipotassium magnesium salt dihydrate
CAS 15708-48-2 · C10H12N2O8K2Mg·2H2O -
EDTA diammonium salt hydrate
CAS 20824-56-0 · HO2CCH2N(CH2CO2NH4)CH2CH2N(CH2CO2NH4)CH2CO2H·H2O -
EDTA magnesium disodium salt hydrate
CAS 14402-88-1 · C10H12MgN2Na2O8 · xH2O -
EDTA (Ethylenediamine tetraacetic acid)
CAS 60-00-4 · (HO2CCH2)2NCH2CH2N(CH2CO2H)2 -
EDTA tripotassium salt dihydrate
CAS 65501-24-8 · (KOOCCH2)2NCH2CH2N(CH2COOK)CH2COOH · 2H2O -
EDTA trisodium salt dihydrate
CAS 150-38-9 · C10H13N2O8Na3·2H2O -
EDTA disodium salt dihydrate
CAS 6381-92-6 · C10H14N2Na2O8 -
EDTA dipotassium salt dihydrate
CAS 25102-12-9 · C2H4N2(CH2COOH)2(CH2COOK)2 · 2H2O -
EDTA tripotassium salt
CAS 17572-97-3 · C10H13K3N2O8 -
HEDTA-Trisodium Salt 41% in H2O
CAS 139-89-9 · C10H15N2Na3O7 -
EDTA tetrasodium salt dihydrate
CAS 10378-23-1 · C10H12N2O8Na4·2H2O -
EDTA ferric sodium salt
CAS 15708-41-5 · C10H12FeN2NaO8
FAQ
What grade of Tris should I use for PCR?
Molecular-biology grade (DNase/RNase-free) is recommended. ACS-grade also works for routine PCR but lacks the certified nuclease-free testing.
What MgCl₂ concentration is best?
1.5–2.0 mM is the standard starting point. Optimize between 1 and 4 mM for tricky templates — more Mg²⁺ relaxes specificity, less Mg²⁺ tightens it.
Does Molekula supply DNase/RNase-free buffer salts?
Yes — many products are available as molecular-biology grade with nuclease testing on the CoA. Request the molecular-biology grade when quoting.
Need a custom grade or pack size?
Talk to a chemist — we run process chemistry from grams up to multi-kilogram campaigns.
Get a quote