Anwendung
Cell culture & media
Sterile-grade salts, buffers, and supplements for mammalian and microbial culture.
Cell culture relies on a balanced salt solution (PBS, HBSS, DPBS), a pH buffer (HEPES, sodium bicarbonate), and supplements (L-glutamine, sodium pyruvate, glucose) added to base media (DMEM, RPMI). Molekula supplies the underlying reagents in cell-culture-tested grades with low endotoxin specifications on request.
Was enthält dieses Workflow
- Salt solutions
- NaCl, KCl, CaCl₂, MgSO₄, KH₂PO₄, Na₂HPO₄.
- pH buffers
- HEPES (independent of CO₂), sodium bicarbonate (CO₂-buffered).
- Supplements
- L-glutamine, sodium pyruvate, D-glucose, β-mercaptoethanol, non-essential amino acids.
- Antibiotics
- Penicillin G, streptomycin sulfate, gentamicin sulfate.
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Häufig gestellte Fragen
Why use HEPES instead of bicarbonate buffer?
HEPES holds pH stable without CO₂, so it works on the bench during microscopy or short manipulations outside the incubator. Cells in long-term culture need bicarbonate + CO₂ for normal physiology.
What grade is required for cell culture?
Cell-culture tested grade with low endotoxin (typically <1 EU/mg) is recommended. Request endotoxin-tested grade when quoting.
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